Dwiyati Pujimulyani
Mercu Buana University, Indonesia
Title: In vitro assay of antioxidant and antidiabetic potency white saffron (curcuma mangga val.) extract and its fractions
Biography
Biography: Dwiyati Pujimulyani
Abstract
Diabetes is known as the most common endocrinal disorder indicated by hyperglycemia and long term complications. Oxidative stress and excess of free radicals have been documented in diabetes occurence. Investigation of natural antidiabetic agents and antioxidants with less side effect is therefore needed. Antidiabetic and antihypertensive activities of white saffron (Curcuma mangga) have been reported. In this study (in vitro method), antidiabetic activity of four fractions of C. mangga extract (water, hexane, ethyl acetate, and buthanol fraction) was measured by α/β glucosidase activity assay, while antioxidant activity of those fractions was measured using nitrite oxide (NO) and H2O2-scavenging activity assay. These fractions were also compared to antidiabetic drug, acarbose, as control. Hexane fraction of C. mangga extract showed the highest α-glucosidase inhibitory activity (68.29%; IC50 = 183.21 µg/mL). Ethyl acetate fraction of C. mangga extract showed the highest β-glucosidase inhibitory (81.58%; IC50 = 163.29 µg/mL) and H2O2-scavenging activity (135.69%; IC50 = 265.66 µg/mL). However, acarbose exhibited the highest NO-scavenging activity (74.75%; IC50 = 165.92 µg/mL) compared to all fractions of extract. To conclude, C. mangga extract could be used as alternative in the development of antidiabetic medicine.