Heba Abdel-Hady
Theodor Bilharz Research Institute, Egypt
Title: GC-MS analysis, antioxidant and cytotoxic activities of Mentha spicata
Biography
Biography: Heba Abdel-Hady
Abstract
Mentha spicata medicinal properties are well known. In this study, total phenolic and flavonoid contents and the antioxidant activity of the methanol extract of Mentha spicata were determined as well as evaluation of the cytotoxic activity of it. Also, the identification of some bioactive compounds in the plant was analyzed. The antioxidant activity was determined by two methods, DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2-2'Δazinobis (3-ethylbenzthiazoline-6- sulphonic acid) while, the cytotoxic assay was determined by MTT assay on HepG-2 (human hepatocellular carcinoma) and MTC-116 (human colon carcinoma). Regarding to the chemical identification of methanol extract was carried out by GC-MS analysis. The results proved that Mentha spicata has high total phenolic and flavonoid contents (388.20±2.38 mg GAE/g of extract & 204.01±17.93 mg RE/g of extract) respectively. Also, exhibited promising antioxidant activity by DPPH & ABTS (IC50=51.13±1.29 μg/ml & 184.31±0.81 μg/ml) respectively. The methanol extract of the plant showed a good cytotoxic effect on HepG2 and HTC-116 (IC50=25.2±3.6 μg/ml & 62.1±4.9 μg/ml) respectively. GC-MS analysis of the methanol extract of Mentha spicata showed 43 oxygenated hydrocarbon compounds. The major ones are hexadecanoic acid, methyl ester (palmitic acid ester) (31.51%) followed by 9,12,15-octadecatrienoic acid, methyl ester (CAS) (methyl linolenate) (22.10%), 2-Pentadecanone, 6,10,14-trimethyl-(CAS) (6.82%), phytol (6.20%), 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (6.18%), hexadecanoic acid (palmitic acid) (5.95%) and methyl stearate (4.49%). The result demonstrated that Mentha spicata is a potential antioxidant and anticancer agents.