Mokhtar M Mabrouk
Tanta University, Egypt
Title: Simultaneous determination of pantoprazole and ketorolac by Reversed phase liquid chromatography in bulk, laboratory prepared mixture and spiked human plasma
Biography
Biography: Mokhtar M Mabrouk
Abstract
A highly sensitive, simple and accurate reversed phase liquid chromatographic method has been developed for determination of pantoprazole and ketorolac in their laboratory prepared mixture and spiked human plasma. This method involves separation of pantoprazole and ketorolac on Thermo ODS Hypersil C18 reversed phase column (4.6×250 mm, particle size 5 μm) using a mobile phase consisting of methanol:phosphate buffer (50 mM; pH 5.5) in a ratio of 60:40 (v/v). The flow rate was 1 mL/min with UV detection at 250 nm. The calibration curves were linear from 0.5 to 100 μg/ml for pantoprazole and from 0.5 to 50 μg/ml for ketorolac. The mean % recoveries for mixtures of both drugs at a ratio 2:1 were found to be 99.29±0.43 and 99.32±0.21 for pantoprazole and ketorolac, respectively using the proposed HPLC method. By applying the method to spiked human plasma using the same optimized chromatographic conditions, the calibration curves were linear from 2 to 70 μg/mL for pantoprazole and from 1 to 60 μg/ml for ketorolac. The mean % recoveries for mixtures of both drugs were found to be 100.08±1.92 and 100.25±2.37 for pantoprazole and ketorolac respectively. The developed methods were successfully applied for the determination of pantoprazole and ketorolac in laboratory prepared mixtures containing all possible excipients present in the tablet dosage form.