Miroslav Pohanka has completed his graduation in Chemistry at Masaryk University Brno, Czech Republic in 2003; Doctor of Natural Science in Biochemistry at Masaryk University in 2006 and PhD in Biochemistry in 2008. He is an Associate Professor in Toxicology at University of Defense, Czech Republic in 2012 and Doctor of Sciences in Analytical Chemistry at Academy of Sciences, Czech Republic in 2014 and Professor in Analytical Chemistry at University of Pardubice, Czech Republic in 2016. He is an Author of more than 200 papers in journals with IF and his works were more than 2000 times cited according Web of Science.
Albumin is a protein serving as a standard marker in biochemistry which is assayed in the blood, blood serum or blood plasma. It serves as a marker in liver function test but its diagnostics importance has more applications. Currently, albumin can be determined by standard immunochemical like ELISA and spectral methods. In this study, immunosensor based on the piezoelectric 10 MHz Quartz Crystal Microbalance (QCM) was constructed to determine albumin in levels corresponding with expected concentration biological samples. The immunosensor contained an antibody specific to albumin interdigitated on a gold electrode of QCM by protein A. The immunosensor was used for the determination of albumin and limit of detection 0.234 mg/ml was reached in the calibration. The limit of detection is lower than the expected albumin plasma level which is 35–55 mg/ml. The immunosensor assay exerted full correlation with the standard ELISA and the immunosensor had also good long-term stability lasting for at least two months. The determination of albumin is a tool suitable for practical use in field therapy or home care conditions. No specific treatment of samples is necessary but specificity and sensitivity typical for ELISA is reached.
Nino Fuchs is a PhD student at the University of Rijeka, Department of Biotechnology. He is appointed as a MD at University Hospital Centre Zagreb, Croatia. His professional interest is focused on plastic and reconstructive surgery. His research interest is focused on the interactions of antineoplastic drugs with herbal supplements on animal experimental models.
Irinotecan (IRI) represents one of the most important antineoplastic drugs used in the chemotherapy of metastatic colorectal cancer. Its use is accompanied by various adverse effects such as diarrhoea, myelosuppression and hepatotoxicity. To diminish side-effects, some cancer patients use Cannabis sativa preparations, rich on delta-9-tetrahydrocannabinol (THC). The potentially harmful interactions resulting from co-administration of chemotherapy with biologically based complementary therapies have not yet been explained well, which motivated us to conduct a study on a Wistar rat model using a THC dose equal to the one found in commonly used illicit preparations. Male rats were concomitantly exposed to IRI (at 100 mg/kg b.w, administered once i.p.) and THC (administered repeatedly for 3 and 7 days per os at 7 mg/kg b.w). Single IRI, THC and control groups were studied in parallel. We estimated changes in brain weight caused by the treatments and evaluated the level of primary DNA damage in the brain cells of exposed and control rats using alkaline comet assay. Combined treatment slightly diminished brain weights compared to controls after both exposure times. The 3-day treatment resulted in significantly increased levels of DNA damage in brain cells of rats given single IRI, which worsened after concomitant exposure to THC. Single THC caused a minor increase of DNA damage compared to control rats. After the 7-day treatment, levels of DNA damage in rats given single IRI and IRI+THC slightly lowered, but were still significantly higher than in control rats. Prolonged exposure to THC resulted in a further increase of DNA damage. The obtained results speak against the concomitant use of THC with IRI, due to the possible enhancement of brain damage, but these findings have to be proven in forthcoming studies using much wider dose ranges of both compounds.